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KMID : 0385020040200020200
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Korean Journal of Laboratory Animal Science
2004 Volume.20 No. 2 p.200 ~ p.207
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N-ethyl-N-nitrosourea mutagenesis: Development of Disease Animal Model
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Cho Kyu-Hyuk
Cho Jae-Woo
Yoon Seok-Joo
Song Chang-Woo
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Abstract
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Stepping into the post genome era, geneticists is focusing their studies on gene function and its role in mechanism of human disease. N-ethyl-N-nitrosourea (ENU) mutagenesis and screening for altered phenotypes have been used effectively in many model mice to identify mutation in genes that control biological processes. ENU is currently one of the most powerful mutagen for the production of mutant in mice. This review includes 1) the principle of mutation by ENU treatment, 2) the process of mutagenesis, 3) screening of phenotypes, 4) mating methods of phenodeviants, breeding methods of high technology and cryopreservation, 5) result of the inside and outside of the country mutant mice development, 6) mapping and mutation analysis of cause genes, and 7) prospect in the future.
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KEYWORD
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ENU mutagenesis, Phenodeviant, Mutant, Mouse
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